Establishment of PC12 cell line stably expressing heteromultimeric complex of P2X2 and P2X3 subtypes
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چکیده
منابع مشابه
Establishment a CHO Cell Line Expressing Human CD52 Molecule
Background: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells...
متن کاملEstablishment and characterization of methylmercury-resistant PC12 cell line.
Methylmercury (MeHg)-resistant sublines of rat pheochromocytoma (PC12) cells were isolated by repeated exposure to stepwise increased concentrations of MeHg. One of the sublines (PC12/TM) showed an 8- to 10-fold increase in resistance to MeHg compared with parent PC12 cells on the basis of the concentration required for 50% inhibition (IC50) of growth. PC12/TM cells accumulated smaller amounts ...
متن کامل[Construction of pcDNA3-HERG-G572R expression vector and establishment of a cell line stably expressing HKE-HERG-G572R].
OBJECTIVE To construct the pcDNA3-HERG-G572R expression vector and establish a cell line stably expressing HKE-HERG-G572R. METHODS HERG-G572R mutant fragment was constructed by over-lap extension PCR and validated by DNA sequencing. The HKE-HERG-G572R expression vector was constructed and transfected into HEK293 cells to obtain a cell line stably expressing HKE-HERG-G572R. RESULTS The pcDNA...
متن کاملestablishment a cho cell line expressing human cd52 molecule
background: cd52 is a small glycoprotein with a gpi anchor at its c-terminus. cd52 is expressed by normal and malignant t and b lymphocytes and monocytes. there are detectable amounts of soluble cd52 in plasma of patients with cll and could be used as a tumor marker. although the biological function of cd52 is unknown but it seems that cd52 may be involved in migration and activation of t-cells...
متن کاملInhibitory role of supraspinal P2X3/P2X2/3 subtypes on nociception in rats
Extracellular ATP is known to mediate synaptic transmission as a neurotransmitter or a neuromodulator via ionotropic P2X and metabotropic P2Y receptors. Several lines of evidence have suggested that ATP facilitates pain transmission at peripheral and spinal sites via the P2X receptors, in which the P2X3 subtype is considered as an important candidate for the effect. Conversely, we previously fo...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1999
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)34580-9